Pentobarbital Immunoassay, Serum/Plasma

CPT: 80299

Synonyms

Pentothal®


Test Includes

Pentobarbital


Expected Turnaround Time

1 day


Related Information

In-house validated method


Specimen Requirements


Specimen

Serum OR Plasma


Volume

1 mL


Minimum Volume

300 μL


Container

Red-top (no additive) tube or green-top (heparin) tube or lavender top (EDTA) tube. Serum/plasma transfer tube. Gel-barrier tubes are not recommended.


Collection

Serum or plasma should be separated from cells within two hours of venipuncture. Submit serum or plasma in a plastic transport tube.


Storage Instructions

Submission/transport (<3 days): Room temperature. For storage beyond three days, specimen should be refrigerated or frozen.


Causes for Rejection

Gel-barrier tubes


Test Details


Use

STAT measurement of pentobarbital in patients with medically induced coma.


Methodology

Immunoassay


Reference Interval

1.0 - 5.0 µg/mL; Pentobarbital concentrations as high as 50 µg/mL may be required to induce therapeutic coma.