Diphenhydramine, serum or plasma

CPT: 80299

Synonyms

Benadryl®


Expected Turnaround Time

7 days


Related Information

In-house validated method


Specimen Requirements


Specimen

Serum or plasma


Volume

3 mL


Minimum Volume

0.6 mL


Container

Red-top (no additive) tube or green-top (heparin) tube. Gel-barrier tubes are not recommended.


Collection

Transfer separated serum or plasma to a plastic transport tube.


Storage Instructions

Submission/transport (<3 days): Room temperature. For storage beyond three days, specimen should be refrigerated or frozen.


Test Details


Use

Therapeutic Drug Management


Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)


Reference Interval

30 - 50 ng/mL; The stated reference range is the range where a significant antihistaminic effect is expected without significant sedation. When sedation is desired, the therapeutic range would be approximately 50 - 300 ng/mL.